Goat polyclonal antibody to ZO1 Tight Junction Protein.
Applications
ELISA, IF
Dilutions
ELISA: 1:64,000, IF: 10µg/ml
Reactivity
Human
Immunogen
Synthetic peptide corresponding to the internal region of human ZO1 Tight Junction Protein.
Sequence
C-PKPTSQNQFSEHDKT
Host
Goat
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 µg/ml
Product Form
Liquid
Formulation
Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
Tight junction protein 1, Tight junction protein ZO-1, Tight junction protein ZO1, TJP1, zo-1, Zo1, ZO1_HUMAN, Zona occludens 1, Zona occludens 1 protein, Zona occludens protein 1, Zonula occludens 1 protein, Zonula occludens protein 1
Immunofluorescence - Anti-ZO1 Tight Junction Protein Antibody (A82887)
ZO1 Tight Junction Protein expression in A431 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-ZO1 Tight Junction Protein Antibody (A82887) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cell junction staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.
Immunofluorescence - Anti-ZO1 Tight Junction Protein Antibody (A82887)
ZO1 Tight Junction Protein expression in U251 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-ZO1 Tight Junction Protein Antibody (A82887) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic and plasma membrane staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.
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