Rabbit polyclonal antibody to YB1 (phospho Ser102).
Specificity
This antibody detects endogenous levels of YB1 only when phosphorylated at Ser102.
Applications
WB, ELISA
Dilutions
WB: 1:500-1:1000, ELISA: 1:40000
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide derived from human YB1 around the phosphorylation site of Ser102 (amino acids 68-117).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.
Molecular Weight
35kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
CBF-A, CCAAT-binding transcription factor I subunit A, DBPB, DNA-binding protein B, EFI-A, Enhancer factor I subunit A, NSEP1, Nuclease-sensitive element-binding protein 1, Y-box transcription factor, Y-box-binding protein 1, YB-1, YBX1
Western Blot - Anti-YB1 (phospho Ser102) Antibody (A94245)
Western blot analysis of lysates from HepG2 cells treated with PMA 125ng/ml 15' using Anti-YB1 (phospho Ser102) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.
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