Rabbit polyclonal antibody to YB1 (Phospho-Ser102)
Specificity
The antibody detects endogenous levels of YB1 only when phosphorylated at serine 102.
Applications
WB
Dilutions
Western blotting: 1:500~1:1000
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of Serine 102(L-R-S(p)-V-G) derived from Human YB1.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20˚C / 1 year
Synonyms
CBF-A, CCAAT-binding transcription factor I subunit A, DBPB, DNA-binding protein B, EFI-A, Enhancer factor I subunit A, NSEP1, Nuclease-sensitive element-binding protein 1, Y-box transcription factor, Y-box-binding protein 1, YB-1, YBX1
Western blot analysis of extracts from HepG2 cells treated with PMA using YB1 (Phospho-Ser102) Antibody #11819.The lane on the right is treated with the antigen-specific peptide.
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