Anti-YB1 Antibody [ARC0797] (A305945)

Name: Anti-YB1 Antibody [ARC0797]
Brand: Antibodies.com
SKU: A305945
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC0797] antibody to YB1 for WB, IHC, ICC/IF, IP and ChIP.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Specifications

Name

Anti-YB1 Antibody [ARC0797]

Description

Rabbit monoclonal [ARC0797] antibody to YB1.

Applications

WB, IHC, ICC/IF, IP, ChIP

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000, ChIP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 225-324 of human YB-1/YBX1 (P67809).

Sequence

GAGEQGRPVRQNMYRGYRPRFRRGPPRQRQPREDGNEEDKENQGDETQGQQPPQRRYRRNFNYRRRRPENPKPQDGKETKAADPPAENSSAPEAEQGGAE

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0797

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

49 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

CBF-A, CCAAT-binding transcription factor I subunit A, DBPB, DNA-binding protein B, EFI-A, Enhancer factor I subunit A, NSEP1, Nuclease-sensitive element-binding protein 1, Y-box transcription factor, Y-box-binding protein 1, YB-1, YBX1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (10)

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Western Blot - Anti-YB1 Antibody [ARC0797] (A305945)

Western blot analysis of extracts of HeLa cells, using Anti-YB1 Antibody [ARC0797] (A305945) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Western Blot - Anti-YB1 Antibody [ARC0797] (A305945)

Western blot analysis of extracts of various cell lines, using Anti-YB1 Antibody [ARC0797] (A305945) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Immunohistochemistry - Anti-YB1 Antibody [ARC0797] (A305945)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-YB1 Antibody [ARC0797] (A305945) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-YB1 Antibody [ARC0797] (A305945)

Immunohistochemistry analysis of paraffin-embedded human oophoroma using Anti-YB1 Antibody [ARC0797] (A305945) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.