Anti-YAP1 (phospho Ser397) Antibody (A305557)

Name: Anti-YAP1 (phospho Ser397) Antibody
Brand: Antibodies.com
SKU: A305557
Price: 485.0 USD
Availability: InStock

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$485

Rabbit polyclonal antibody to YAP1 (phospho Ser397) for WB, ICC/IF and ELISA.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-YAP1 (phospho Ser397) Antibody

Description

Rabbit polyclonal antibody to YAP1 (phospho Ser397).

Applications

WB, ICC/IF, ELISA

Positive Control

NIH/3T3 treated by Sorbitol, C6 treated by Sorbitol.

Dilutions

WB: 1:500-1:1,000, ICC/IF: 1:50-1:200, ELISA: 1 µg/ml (starting concentration)

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide phosphorylated at S397 of human YAP1.

Sequence

DESTD

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

75 kDa

Predicted MW

54 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Protein yorkie homolog, Transcriptional coactivator YAP1, YAP65, Yes-associated protein 1, Yes-associated protein YAP65 homolog

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Western Blot - Anti-YAP1 (phospho Ser397) Antibody (A305557)

YAP1 expression in various lysates analyzed by western blot. Both cell types were treated with Sorbitol (0.2M) at 37°C for 60 minutes following serum starvation overnight. Primary antibody incubation was performed for 1 hour on 25ug protein per lane with Anti-YAP1 (Phospho S397) Antibody (A305557) at a dilution of 1:1000 and detected with chemiluminescence.

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Immunofluorescence - Anti-YAP1 (phospho Ser397) Antibody (A305557)

YAP1 expression in HeLa cells (treated with CA) and HeLa cells (untreated) analyzed by immunofluorescence. Staining was performed with Anti-YAP1 (Phospho S397) Antibody (A305557) at a dilution of 1:100 followed by Cy3-conjugated Goat anti-Rabbit IgG (H+L)secondary antibody at a dilution of 1:500. Nuclei were stained with DAPI (blue).

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