Anti-XBP1 Antibody (A309876)

Name: Anti-XBP1 Antibody
Brand: Antibodies.com
SKU: A309876
Price: 355.0 USD
Availability: InStock

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$355

Rabbit polyclonal antibody to XBP1 for WB and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-XBP1 Antibody

Description

Rabbit polyclonal antibody to XBP1.

Applications

WB, ICC/IF

Dilutions

WB: 1:100-1:500, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human XBP1S.

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

60 kDa / 55 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Tax-responsive element-binding protein 5, TREB-5, TREB5, X-box-binding protein 1, XBP-1, XBP2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (5)

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Western Blot - Anti-XBP1 Antibody (A309876)

Western blot analysis of various lysates, using Anti-XBP1 Antibody (A309876) at 1:400 dilution. HeLa, C2C12 and cells were treated by TSA (1 uM) at 37°C for 18 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 20s.

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Western Blot - Anti-XBP1 Antibody (A309876)

Western blot analysis of Rat lung, using Anti-XBP1 Antibody (A309876) at 1:400 dilution. HeLa, C2C12 and cells were treated by TSA (1 uM) at 37°C for 18 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 60s.

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Immunofluorescence - Anti-XBP1 Antibody (A309876)

Immunofluorescence analysis of Hep-G2 cells using Anti-XBP1 Antibody (A309876) at a dilution of 1:20 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Immunofluorescence - Anti-XBP1 Antibody (A309876)

Immunofluorescence analysis of HT-29 cells using Anti-XBP1 Antibody (A309876) at a dilution of 1:20 (40x lens). DAPI was used to stain the cell nuclei (blue).