Wnt3 expression in HEK293 cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-Wnt3 Antibody (A84213) at 1µg/ml and detected by chemiluminescence.
Wnt3 expression in Human Breast analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-Wnt3 Antibody (A84213) at 2µg/ml and revealed with horseradish peroxidase (HRP).
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