Anti-VPS35 Antibody [7E4] (A305036)

Immunoprecipitation analysis of mouse embryonic fibroblast using Anti-VPS35 Antibody [7E4] (A305036). 500 µL cell culture supernatants were incubated with 10 µL of Protein A/G resin beads for 1 hour at 4°C. A305036 clone 7E4 depletes virtually all of the VPS35 from the A549 cell extract..

Western blot analysis of human SH-SY5Y lysates showing detection of 91.7 kDa VPS35 protein using Anti-VPS35 Antibody [7E4] (A305036) at 1:1,000 for 2 hours at room temperature with shaking. Lane 1: Molecular Weight Ladder. Lane 2: SH-SY5Y. Load: 10 μg. Block: 5% Skim Milk powder in TBST. The secondary antibody used was Goat anti-mouse IgG:HRP at 1:4,000 for 1 hour at room temperature with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 minutes in room temperature. Predicted/Observed Size: 91.7 kDa.

Western blot analysis of Human, mouse A549, MEF showing detection of VPS35 protein using Anti-VPS35 Antibody [7E4] (A305036) at 1:5 (tissue culture supernatant). Lane 1: Molecular Weight Ladder. Lane 2: VPS35 KO A549 cells. Lane 3: mouse embryonic fibroblast cells.. Load: 8 µg each A549 and MEF. The secondary antibody used was Donkey anti-mouse IRDye 800CW at 1:25,000 in TBS-T.

Immunocytochemistry/Immunofluorescence analysis of human A549 cells, using Anti-VPS35 Antibody [7E4] (A305036), at 1:5 (tissue culture supernatant). The secondary antibody used was Donkey anti-mouse: Alexa Fluor 594 at 1:4,000 in 0.2% BSA PBS. Counterstain: DAPI. Localization: Vesicles. A) VPS35 KO A549 cells B) WT A549 cells.