Anti-VPS35 Antibody [10A8] (A305035)

Name: Anti-VPS35 Antibody [10A8]
Brand: Antibodies.com
SKU: A305035
Price: 580.0 USD
Availability: InStock

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$580

Mouse monoclonal [10A8] antibody to VPS35 for WB, ICC/IF, IP and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Specifications

Name

Anti-VPS35 Antibody [10A8]

Description

Mouse monoclonal [10A8] antibody to VPS35.

Applications

WB, ICC/IF, IP, IHC

Dilutions

WB: 1:1,000, ICC/IF: 1:200, IP: 1:200, IHC: 1:100

Reactivity

Human, Mouse, Rat

Immunogen

Full length recombinant human VSP35.

Host

Mouse

Clonality

Monoclonal

Clone ID

10A8

Isotype

IgG2a

Conjugate

Unconjugated

Purification

Protein G purification.

Concentration

1 mg/ml

Molecular Weight

~92 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

hVPS35, Maternal-embryonic 3, MEM3, Vacuolar protein sorting-associated protein 35, Vesicle protein sorting 35

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (8)

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Immunofluorescence - Anti-VPS35 Antibody [10A8] (A305035)

Immunocytochemistry/Immunofluorescence analysis of human A549 WT, VPS35 KO cells, using Anti-VPS35 Antibody [10A8] (A305035). The secondary antibody used was Donkey Anti-Mouse AlexaFluor 594. Clone can detect VPS35 at 1/2000 concentration.

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Immunoprecipitation - Anti-VPS35 Antibody [10A8] (A305035)

Immunoprecipitation analysis of mouse embryonic fibroblast using Anti-VPS35 Antibody [10A8] (A305035). Three amounts of A305035 (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4°C and next incubated with 250ug of A549 lysate for 2 hours at 4°C.

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Immunoprecipitation - Anti-VPS35 Antibody [10A8] (A305035)

Immunoprecipitation analysis of mouse embryonic fibroblast using Anti-VPS35 Antibody [10A8] (A305035).

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Western Blot - Anti-VPS35 Antibody [10A8] (A305035)

Western blot analysis of Human, mouse A549, MEF showing detection of VPS35 protein using Anti-VPS35 Antibody [10A8] (A305035) at 1:5 (tissue culture supernatant). Lane 1: Molecular Weight Ladder. Lane 2: VPS35 KO A549 cells. Lane 3: mouse embryonic fibroblast cells.. Load: 8 µg each A549 and MEF. The secondary antibody used was Donkey anti-mouse IRDye 800CW at 1:25,000 in TBS-T.