Anti-VPS15 Antibody (A297489)

Name: Anti-VPS15 Antibody
Brand: Antibodies.com
SKU: A297489
Price: 490.0 USD
Availability: InStock

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$490

Rabbit polyclonal antibody to VPS15 for WB and IP.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 6-9 business days.

Specifications

Name

Anti-VPS15 Antibody

Description

Rabbit polyclonal antibody to VPS15.

Applications

WB, IP

Dilutions

IP: 2-5 µg/1 mg lysate, WB: 1:2,000-1:10,000

Reactivity

Human, Mouse

Immunogen

Synthetic peptide within amino acids 1300-1358 of human VPS15 (NP_055417. 1).

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Antigen affinity purification.

Concentration

200 µg/ml

Product Form

Liquid

Formulation

Supplied in Tris-Buffered Saline with 0.1% BSA and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

General Notes

This antibody was affinity purified using the immunising peptide immobilized on solid support. Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1. 4 equals 1.0 mg of IgG.

Synonyms

Phosphoinositide 3-kinase adaptor protein, Phosphoinositide 3-kinase regulatory subunit 4, PI3-kinase p150 subunit, PI3-kinase regulatory subunit 4, PIK3R4

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Western Blot - Anti-VPS15 Antibody (A297489)

Samples: Whole cell lysate from HeLa (5, 15 and 50 µg ), HEK293T (T; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Anti-VPS15 Antibody (A297489) was used at 0.04 µg/ml. Detection: Chemiluminescence with exposure time of 3 minutes.

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Immunoprecipitation - Anti-VPS15 Antibody (A297489)

Samples: Whole cell lysate from HeLa (1 mg for IP, 20% of IP loaded) cells. Antibodies: Anti-VPS15 Antibody (A297489) was used for WB at 1 µg/ml and used for IP at 3 µg/mg lysate. VPS15 was also immunoprecipitated using another rabbit anti-VPS15 antibody , which recognizes an upstream epitope. Detection: Chemiluminescence with exposure time of 30 seconds.

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