Anti-Von Willebrand Factor Antibody [C01-1F2] (A279925)

Anti-Von Willebrand Factor Antibody [C01-1F2]

Mouse monoclonal [C01-1F2] antibody to Von Willebrand Factor.

This antibody recognises von Willebrand factor (vWF) a multifunctional glycoprotein that plays essential roles in primary and secondary homeostasis, mediates platelet adhesion and is a carrier of coagulation factor VIII. vWF is present in blood plasma, the subendothelial matrix, platelet granules (a granules) and endothelial cells. In endothelial cells, the vWF is stored in mature Weibel-Palade bodies before it is secreted into the plasma. vWF plasma levels are influenced by genetic, pathological, hormonal and environmental interactions. Upon vascular injury, vWF mediates the anchoring of platelets to the subendothelium leading to the formation of a platelet plug and the arrest of bleeding. Due to its acute release by the activated epithelium, vWF has been considered a marker of inflammation in various pathologies. However there is evidence that vWF is able to play a direct role in inflammation by recruiting leukokcytes to sites of inflammation. The purified Mouse anti Human vWF antibody, clone C01-1F2 (MCA6288GA) can be used as a capture antibody in a sandwich ELISA with the biotinylated Mouse anti Human vWF antibody, clone E01-1C8 (MCA6289B) as the detection antibody.

ELISA: 1 µg/ml

Human

CHO derived recombinant full length human vWF (Met1-Lys2813).

Mouse

Monoclonal

C01-1F2

IgG1

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Human vWF antibody, clone C01-1F2, recognizes von Willebrand factor (vWF) a multifunctional glycoprotein that plays essential roles in primary and secondary homeostasis, mediates platelet adhesion and is a carrier of coagulation factor VIII (Yasar et al. 2018). vWF is present in blood plasma, the subendothelial matrix, platelet granules (a granules) and endothelial cells (Kawecki et al. 2017). In endothelial cells, the vWF is stored in mature Weibel-Palade bodies before it is secreted into the plasma (Yasar et al. 2018). vWF plasma levels are influenced by genetic, pathological, hormonal and environmental interactions (Swystun & Lillicrap 2018). Upon vascular injury, vWF mediates the anchoring of platelets to the subendothelium leading to the formation of a platelet plug and the arrest of bleeding. Due to its acute release by the activated epithelium, vWF has been considered a marker of inflammation in various pathologies. However there is evidence that vWF is able to play a direct role in inflammation by recruiting leukokcytes to sites of inflammation (Kawecki et al. 2017). The purified Mouse anti Human vWF antibody, clone C01-1F2 (MCA6288GA) can be used as a capture antibody in a sandwich ELISA with the biotinylated Mouse anti Human vWF antibody, clone E01-1C8 (MCA6289B) as the detection antibody.

F8VWF