Recombinant Anti-VISTA Antibody [DMC299] - BSA and Azide free (A318768)

Name: Anti-VISTA Antibody [DMC299] - BSA and Azide free
Brand: Antibodies.com
SKU: A318768
Price: 665.0 USD
Availability: InStock

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$665

Recombinant chimeric monoclonal [DMC299] antibody to VISTA for Flow Cytometry.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-8 business days.

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Recombinant Antibody

Specifications

Name

Anti-VISTA Antibody [DMC299] - BSA and Azide free

Description

Recombinant chimeric monoclonal [DMC299] antibody to VISTA.

Applications

Flow Cytometry

Dilutions

Flow Cytometry: 1:100

Reactivity

Human

Host

Rabbit / Human

Clonality

Monoclonal

Clone ID

DMC299

Isotype

IgG1

Conjugate

Unconjugated

Purification

Affinity chromatography.

Concentration

Reconstitution dependent.

Product Form

Lyophilized

Reconstitution

Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.

Formulation

Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.

Storage

Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.

General Notes

This antibody is a recombinant rabbit chimeric monoclonal antibody containing human IgG1 Fc.

Synonyms

C10orf54, Platelet receptor Gi24, Sisp-1, SISP1, Stress-induced secreted protein-1, V-set domain-containing immunoregulatory receptor, V-set immunoregulatory receptor, V-type immunoglobulin domain-containing suppressor of T-cell activation, VSIR

Suitable Secondaries

See all Anti-Human IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Flow Cytometry - Anti-VISTA Antibody [DMC299] - BSA and Azide free (A318768)

Flow cytometry analysis of Anti-VISTA Antibody [DMC299] - BSA and Azide free (A318768) on Expi293 cells transfected with human B7H5 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).

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Flow Cytometry - Anti-VISTA Antibody [DMC299] - BSA and Azide free (A318768)

Flow cytometry analysis of antigen binding of Anti-VISTA Antibody [DMC299] - BSA and Azide free (A318768). (A) Anti-VISTA Antibody [DMC299] - BSA and Azide free (A318768) does not bind to 293T cells that do not express B7-H5. (B) A clear peak shift of Anti-VISTA Antibody [DMC299] - BSA and Azide free (A318768) was seen compared to the control when incubated with B7-H5-expressing THP-1 cells, indicating strong binding of Anti-VISTA Antibody [DMC299] - BSA and Azide free (A318768) to B7-H5. Antibodies were incubated at 5 µg/ml.

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