Recombinant Anti-Vinculin Antibody [VCL/8533R] (A315784)

Anti-Vinculin Antibody [VCL/8533R]

Recombinant rabbit monoclonal [VCL/8533R] antibody to Vinculin.

Focal adhesions are identified as areas within the plasma membrane of tissue culture cells that adhere tightly to the underlying substrate. In vivo, these regions are involved in the adhesion of cells to the extracellular matrix. Paxillin and vinculin are cytoskeletal, focal adhesion proteins that are components of a protein complex which links the Actin network to the plasma membrane. Vinculin binding sites have been identified on other cytoskeletal proteins, including Talin and -Actinin has been shown to link Actin to integrins in the plasma membrane through interactions with the vinculin and Talin complex or by a direct interaction with integrin.

WB, IHC-P

A431, K562, NIH3T3, U-87 or THP-1 cells. Human bladder or testis.

WB: 1-2 µg/ml, IHC: 1-2 µg/ml

Human, Mouse

Recombinant fragment (around Amino Acids 174-322) of human Vinculin (VCL) protein. The exact sequence is proprietary.

Rabbit

Monoclonal

VCL/8533R

kappa

Protein A/G chromatography.

200 µg/ml

117 kDa

Liquid

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Metavinculin, MV, VCL