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Anti-Vinculin Antibody [VCL/3617] (A250291)

Mouse monoclonal [VCL/3617] antibody to Vinculin for IF, Flow Cytometry and IHC-P.

Alternative Formats and ConjugatesAlternative Formats

Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 5-8 business days.
Name
Anti-Vinculin Antibody [VCL/3617]
Description
Mouse monoclonal [VCL/3617] antibody to Vinculin.
Specificity
This antibody recognizes both Vinculin (125kDa) and meta-Vinculin (135kDa). Focal adhesions are identified as areas within the plasma membrane of tissue culture cells that adhere tightly to the underlying substrate. In vivo, these regions are involved in the adhesion of cells to the extracellular matrix. Paxillin and vinculin are cytoskeletal, focal adhesion proteins that are components of a protein complex which links the Actin network to the plasma membrane. Vinculin binding sites have been identified on other cytoskeletal proteins, including Talin and -Actinin has been shown to link Actin to integrins in the plasma membrane through interactions with the vinculin and Talin complex or by a direct interaction with integrin.
Applications
IF, Flow Cytometry, IHC-P
Dilutions
IF: 1-2 µg/ml, Flow Cytometry: 1-2 µg/million cells, IHC-P: 1-2 µg/ml
Reactivity
Human, Mouse, Rat, Bovine, Porcine, Rabbit, Frog, Fish, Bird
Immunogen
Recombinant full-length human Vinculin protein.
Host
Mouse
Clonality
Monoclonal
Clone ID
VCL/3617
Isotype
IgG1
Light Chains
kappa
Conjugate

Unconjugated

Purification
Protein A/G chromatography.
Concentration
200 µg/ml
Molecular Weight
125-135 kDa
Product Form
Liquid
Formulation
Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
General Notes
This monoclonal antibody is also available in a different formulation without BSA and Sodium Azide - Anti-Vinculin Antibody [VCL/3617] - BSA and Azide free (A253471).
Synonyms
Metavinculin, MV, VCL
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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