Anti-Vinculin Antibody (A329959)

Vinculin expression in HeLa cell lysates analyzed by western blot. Primary antibody incubation was performed for 1 hour on 25ug protein per lane with Anti-Vinculin Antibody (A329959) at a dilution of 1:1000 and detected with chemiluminescence.

Vinculin expression in Hela cells analyzed by immunofluorescence. Staining was performed with Anti-Vinculin Antibody (A329959) at a dilution of 1:100 followed by Cy3-conjugated Goat anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:500. Nuclei were stained with DAPI (blue).

Vinculin expression in HepG2 cells analyzed by immunofluorescence. Staining was performed with Anti-Vinculin Antibody (A329959) at a dilution of 1:500 followed by Cy3-conjugated Goat anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:500. Nuclei were stained with DAPI (blue).