Recombinant Anti-Vimentin Antibody [VIM/1937R] (A250314)

Anti-Vimentin Antibody [VIM/1937R]

Recombinant rabbit monoclonal [VIM/1937R] antibody to Vimentin.

This antibody reacts with a 58kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.

Flow Cytometry, IF, WB, IHC-P

Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/ml, WB: 1-2 µg/ml, IHC-P: 1-2 µg/ml

Human, Rat, Horse, Chicken, Bovine, Feline, Canine, Porcine

This antibody does not cross react with Mouse.

Recombinant full-length human Vimentin protein.

Rabbit

Monoclonal

VIM/1937R

Protein A/G chromatography.

200 µg/ml

57-60 kDa

Liquid

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

This monoclonal antibody is also available in a different formulation without BSA and Sodium Azide - Anti-Vimentin Antibody [VIM/1937R] - BSA and Azide free (A253494).

VIM