This antibody reacts with vimentin, a 57 kDa intermediate filament expressed in variety of mesenchymal and mesodermal cell types. Cross-reactivity was found with smooth muscle desmin.
Applications
IHC-P, WB, ICC, Flow Cytometry
Dilutions
IHC: Staining technique: (a) fix cells for 10 min in methanol at -20°C and for 6 min in acetone at -20°C; (b) fix cells directly in methanol for 10 min at -20°C or in acetone for 10 min at -20°C. Positive Control: 3T3 murine Swiss albino fibroblast cell line, RBL rat basophilic leukemia cell line, Flow Cytometry: 1-5 µg/ml; Intracellular staining.
Reactivity
Mammalian
Immunogen
Pellet of porcine brain cold-stable proteins after depolymerization of microtubules.
Host
Mouse
Clonality
Monoclonal
Clone ID
VI-01
Isotype
IgM
Conjugate
Unconjugated
Purification
Purified by differential precipitation and solid-phase chromatography.
Concentration
1 mg/ml
Predicted MW
57 kDa
Product Form
Liquid
Formulation
Supplied in Tris Buffered Saline, pH 8, with 15 mM Sodium Azide.
Western Blot - Anti-Vimentin Antibody [VI-01] (A86296)
Western Blotting analysis of Vimentin in whole cell lysate of LEP-19 human fibroblast cell line (1,3) and 3T3 mouse fibroblast cell line (2,4). Lane 1,2: Immunostaining with Anti-Vimentin Antibody [VI-01]. Lane 3,4: Immunostaining with Anti-Vimentin Antibody [VI-RE/1].
Western Blot - Anti-Vimentin Antibody [VI-01] (A86296)
Immunoprecipitation of vimentin from HeLa cell lysate by Anti-Vimentin Antibody [VI-10] and its detection by Anti-Vimentin Antibody [VI-01]. IgM heavy chain (76-92 kDa) and IgM light chain (25-30 kDa) indicated. Mr of vimentin is 57 kDa. Lr = lysate (reducing conditions). Lnr = lysate (non-reducing conditions). IPr = immunoprecipitate (reducing conditions). IPnr = immunoprecipitate (non-reducing conditions).
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