Anti-Vimentin Antibody (A85421)

Immunofluorescent analysis of HeLa cells stained with Anti-Fibrillarin Antibody (A104320), at a dilution of 1:5,000, in red, and Anti-Vimentin Antibody (A85421), at a dilution of 1:10,000, in green. The blue is DAPI staining of nuclear DNA. Anti-Fibrillarin Antibody (A104320) detects protein expressed in nucleoli of cells, while Anti-Vimentin Antibody (A85421) produces strong staining of cytoplasmic intermediate filaments.

Immunofluorescent analysis of rat embryonic brain section stained with Anti-FABP7 Antibody [2A84] (A270549), at a dilution of 1:500, in green, and co-stained with Anti-Vimentin Antibody (A85421), at a dilution of 1:5,000, in red. Anti-FABP7 Antibody [2A84] (A270549) stains developing astrocytes and radial glia, while Anti-Vimentin Antibody (A85421) reveals cytoplasmic intermediate filaments. Since the subcellular distribution of these two proteins is distinct, their expression patterns overlap but are not identical.

Immunofluorescent analysis of HeLa cells stained with Anti-Annexin VI Antibody [4G3] (A270554), at a dilution of 1:500, in red, and co-stained with Anti-Vimentin Antibody (A85421), at a dilution of 1:2,000, in green. Nuclear DNA is visualised in blue using Hoechst staining. Anti-Annexin VI Antibody [4G3] (A270554) detects protein predominantly expressed in spherical cellular membrane protrusions, known as blebs, while Anti-Vimentin Antibody (A85421) produces strong staining of the intermediate filament network of the cytoskeleton.

Immunofluorescent analysis of HeLa cell culture stained with Anti-MARCKS Antibody [5F9] (A270556), at a dilution of 1:1,000, in red, and co-stained with Anti-Vimentin Antibody (A85421), at a dilution of 1:10,000, in green. Nuclear DNA is visualised in blue using Hoechst staining. Anti-MARCKS Antibody [5F9] (A270556) binds to MARCKS protein expressed in the membrane structures and cytoplasm, while Anti-Vimentin Antibody (A85421) stains the intermediate filaments.

Immunofluorescent analysis of HeLa cells stained with Anti-Annexin V Antibody [6A12] (A270559), at a dilution of 1:1,000, in green, and co-stained with Anti-Vimentin Antibody (A85421), at a dilution of 1:2,000, in red. Nuclear DNA is visualised in blue using Hoechst staining. Hela cells were grown under normal conditions, on the left, or treated with 15mM Pentoxifyline for 24 hours, on the right. Pentoxyifyline activates apoptosis in these cells and also upregulates Annexin A6 expression.

Confocal immunofluorescent analysis of HeLa cells stained with Anti-Fibrillarin Antibody (A104320) at a dilution of 1:5,000 (red) and Anti-Vimentin Antibody (A85421) at a dilution of 1:10,000 (green). Nuclei were stained with DAPI (blue). The antibody detects protein expressed in the nucleoli of cells, while the vimentin antibody produces strong staining of cytoplasmic intermediate filaments.

Neuromics hN2 are human neuronal lineage cells which originate from ArunA Biomedical, and were derived from the human embryonic cell line WA09. We obtained the hN2 Human Neurons Discovery kit from Neuromics, which arrived frozen and on dry ice. We fixed and stained them with Anti-Vimentin Antibody (A85421) (red). Islands of hN2 cells form after 4 days in culture forming beautiful flower like structures. Vimentin is a well established marker of early differentiating neuronal lineage cells, though it is found in many other kinds of cells. Merged from several images taken with a 10X objective lens, this image is also available as a high resolution poster. Nuclei were stained with DAPI (blue).