Anti-Vimentin Antibody (A85420)

Name: Anti-Vimentin Antibody
Brand: Antibodies.com
SKU: A85420
Price: 190.0 USD
Availability: InStock

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$190

Rabbit polyclonal antibody to Vimentin for WB, ICC/IF and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Tags:

Cytoskeleton Marker Neural Stem Cell Marker Radial Glia Marker

Specifications

Name

Anti-Vimentin Antibody

Description

Rabbit polyclonal antibody to Vimentin.

Applications

WB, ICC/IF, IHC

Dilutions

WB: 1:10,000, ICC/IF: 1:5,000, IHC: 1:5,000

Reactivity

Human, Rat, Mouse, Bovine, Porcine, Horse

Immunogen

Recombinant full-length human Vimentin, expressed in and purified from E. coli.

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Molecular Weight

50 kDa

Purity

Whole antiserum.

Product Form

Liquid

Formulation

Supplied as an aliquot of serum with 5mM Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

VIM

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (7)

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Immunofluorescence - Anti-Vimentin Antibody (A85420)

Immunofluorescence analysis of HeLa cells co-stained with Anti-Vimentin Antibody, at a dilution of 1:5,000, in green, and Anti-Actin Antibody (A85388 | 1:500, in red. Blue is DAPI staining of nuclear DNA. The Anti-Vimentin Antibody stains the 10nm or intermediate filament network of the cytoskeleton. The Anti-Actin Antibody labels the submembranous actin-rich cytoskeleton, stress fibers, and bundles of actin associated with cell adhesion sites.

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Immunofluorescence - Anti-Vimentin Antibody (A85420)

View of HeLa cells stained with Anti-Vimentin Antibody (red) and Anti-Lamin A/C Antibody (A85444 | green). DNA is blue. Anti-Vimentin Antibody reveals strong cytoplasmic intermediate filament staining, while Anti-Lamin A/C Antibody reveals strong nuclear lamina staining.

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Western Blot - Anti-Vimentin Antibody (A85420)

Western blot analysis of whole cell lysates using Anti-Vimentin Antibody, at a dilution of 1:5000, in green,: [Lane 1] protein standard (red), [Lane 2] HeLa, [Lane 3] SH-SY5Y, [Lane 4] HEK293, [Lane 5] NIH 3T3 cells. Strong band corresponds to vimentin protein with apparent SDS-PAGE molecular weight of 54 kDa.

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Immunofluorescence - Anti-Vimentin Antibody (A85420)

Immunofluorescent analysis of mixed fibroblast and PC12 pheochromocytoma cell culture stained with Anti-Peripherin Antibody (A85435) at a dilution of 1:5,000 (green) and costained with Anti-Vimentin Antibody (A85420) at a dilution of 1:2,000, (red). Nuclei were stained with DAPI (blue). Anti-Peripherin Antibody (A85435) reveals cytoplasmic filamentous staining in the small PC12 cells, while vimentin antibody stains intermediate filaments in the surrounding fibroblastic cells which do not express peripherin.