Western blot analysis of extracts of various cell lines, using Anti-Vimentin Antibody (A81195) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Western blot analysis of extracts from normal (control) and Vimentin knockout (KO) 293T cells, using Anti-Vimentin Antibody (A81195) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.
Immunofluorescence analysis of U2OS cells using Anti-Vimentin Antibody (A81195) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).
Publishing research using Anti-Vimentin Antibody (A81195)? Please let us know so that we can list the citation on this page.
![Immunocytochemistry - Anti-Vimentin Antibody [VI-10] (A86650) - Antibodies.com](https://cdn.antibodies.com/image/catalog/86/A86652_789.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-Vimentin Antibody [VIM/1937R] - BSA and Azide free (A253494) - Antibodies.com](https://cdn.antibodies.com/image/catalog/253/A253494_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-Vimentin Antibody [VIM/1937R] (A250314) - Antibodies.com](https://cdn.antibodies.com/image/catalog/250/A250314_1.jpg?profile=product_alternative)
![Western Blot - Anti-Vimentin Antibody [ARC0086] (A305554) - Antibodies.com](https://cdn.antibodies.com/image/catalog/305/A305554_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-Vimentin Antibody [V9] (A250310) - Antibodies.com](https://cdn.antibodies.com/image/catalog/250/A250310_1.jpg?profile=product_alternative)
![Western Blot - Anti-Vimentin Antibody [VM452] - BSA and Azide free (A253487) - Antibodies.com](https://cdn.antibodies.com/image/catalog/253/A253487_1.jpg?profile=product_alternative)
![Western Blot - Anti-Vimentin Antibody [VM452] (A250307) - Antibodies.com](https://cdn.antibodies.com/image/catalog/250/A250307_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-Vimentin Antibody [V9] - BSA and Azide free (A253490) - Antibodies.com](https://cdn.antibodies.com/image/catalog/253/A253490_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-Vimentin Antibody [VIM/6576R] (A250313) - Antibodies.com](https://cdn.antibodies.com/image/catalog/250/A250313_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-Vimentin Antibody [rVIM/6575] - BSA and Azide free (A253486) - Antibodies.com](https://cdn.antibodies.com/image/catalog/253/A253486_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-Vimentin Antibody [VIM/6576R] - BSA and Azide free (A253493) - Antibodies.com](https://cdn.antibodies.com/image/catalog/253/A253493_1.jpg?profile=product_alternative)