Anti-Vimentin Antibody (A14084)

Name: Anti-Vimentin Antibody
Brand: Antibodies.com
SKU: A14084
Price: 355.0 USD
Availability: InStock

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$355

Rabbit polyclonal antibody to Vimentin for WB, IHC-P, ICC/IF and ELISA.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Tags:

Cytoskeleton Marker Neural Stem Cell Marker Radial Glia Marker

Specifications

Name

Anti-Vimentin Antibody

Description

Rabbit polyclonal antibody to Vimentin.

Applications

WB, IHC-P, ICC/IF, ELISA

Positive Control

K-562, HeLa, Jurkat, NIH/3T3, A-549.

Dilutions

WB: 1:500-1:2,000, IHC-P: 1:50-1:200, ICC/IF: 1:50-1:200, ELISA: 1 µg/ml (starting concentration)

Reactivity

Human, Mouse, Rat

Immunogen

A recombinant fusion protein corresponding to amino acids 1-200 of human Vimentin.

Sequence

MSTRSVSSSSYRRMFGGPGTASRPSSSRSYVTTSTRTYSLGSALRPSTSRSLYASSPGGVYATRSSAVRLRSSVPGVRLLQDSVDFSLADAINTEFKNTRTNEKVELQELNDRFANYIDKVRFLEQQNKILLAELEQLKGQGKSRLGDLYEEEMRELRRQVDQLTNDKARVEVERDNLAEDIMRLREKLQEEMLQREEAE

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

58 kDa / 57 kDa

Predicted MW

54 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

VIM

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (7)

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Western Blot - Anti-Vimentin Antibody (A14084)

Vimentin expression in various lysates analyzed by western blot. Primary antibody incubation was performed for 1 hour on 25ug protein per lane with Anti-Vimentin Antibody (A14084) at a dilution of 1:1000 and detected with chemiluminescence.

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Western Blot - Anti-Vimentin Antibody (A14084)

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Western Blot - Anti-Vimentin Antibody (A14084)

Vimentin expression in A549 cell lysates analyzed by western blot. Primary antibody incubation was performed for 1 hour on 25ug protein per lane with Anti-Vimentin Antibody (A14084) at a dilution of 1:3000 and detected with chemiluminescence.

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Immunohistochemistry - Anti-Vimentin Antibody (A14084)

Vimentin expression in human lung tissue analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by microwaving in 10 mM PBS buffer, pH 7.2. Staining was performed with Anti-Vimentin Antibody (A14084) at a dilution of 1:100.