Rabbit polyclonal antibody to VEGFR2 (Phospho-Tyr1214)
Specificity
The antibody detects endogenous level of VEGFR2 only when phosphorylated at tyrosine 1214.
Applications
WB, IHC, IF
Dilutions
Western blotting: 1:500~1:1000 Immunohistochemistry: 1:50~1:100 Immunofluorescence: 1:100~1:200
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of tyrosine 1214 (F-H-Y(p)-D-N) derived from Human VEGFR2.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Predicted MW
230kd
Formulation
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Western blot analysis of extracts from SKOV3 cells using VEGFR2 (Phospho-Tyr1214) Antibody #11085 (Lane 1) and the same antibody preincubated with blocking peptide (Lane2).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using VEGFR2 (Phospho-Tyr1214) Antibody #11085 (left) or the same antibody preincubated with blocking peptide (right).