Anti-VEGFA Antibody (A87633)

Name: Anti-VEGFA Antibody
Brand: Antibodies.com
SKU: A87633
Price: 355.0 USD
Availability: InStock

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$355

Rabbit polyclonal antibody to VEGFA for WB, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-VEGFA Antibody

Description

Rabbit polyclonal antibody to VEGFA.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 100-191 of human VEGFA (NP_001165097.1).

Sequence

SNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPKKDRARQENPCGPCSERRKHLFVQDPQTCKCSCKNTDSRCKARQLELNERTCRCDKPRR

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

15 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

L-VEGF, Vascular endothelial growth factor A, long form, Vascular permeability factor, VEGF, VPF

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-VEGFA Antibody (A87633)

Western blot analysis of extracts of various cell lines, using Anti-VEGFA Antibody (A87633) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Immunohistochemistry - Anti-VEGFA Antibody (A87633)

Immunohistochemistry analysis of paraffin-embedded human lung cancer using Anti-VEGFA Antibody (A87633) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunofluorescence - Anti-VEGFA Antibody (A87633)

Immunofluorescence analysis of NIH/3T3 cells using Anti-VEGFA Antibody (A87633) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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