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Optimize your experiments with our technical guides for Western Blot, ICC/IF, IHC, IP, Flow Cytometry, ChIP, and ELISA >>
Optimize your experiments with our technical guides for Western Blot, ICC/IF, IHC, IP, ChIP, and more >>
Optimize your experiments with our guides for WB, ICC/IF, IHC, IP, and more >>
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Home
Primary Antibodies
VEGFA Antibodies
Anti-VEGFA Antibody (A48157)
Anti-VEGFA Antibody (A48157)
Overview
Specifications
Images
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Best Seller
for "
VEGFA Antibody
"
$405
Rabbit polyclonal antibody to VEGFA for E, WB and IHC.
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Price Match Guarantee
Product Size:
100µl
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Shipping Information
Freight/Packing Charges:
$40
Dispatched from St. Louis, MO.
Lead Time: 6-9 business days.
Specifications
Name
Anti-VEGFA Antibody
Description
Rabbit polyclonal antibody to VEGFA
Specificity
The antibody detects endogenous level of total VEGFA protein.
Applications
E,
WB
,
IHC
Dilutions
ELISA: 1:2000-1:5000 Western blotting: 1:500-1:2000 Immunohistochemistry: 1:25-1:100
Reactivity
Human
Immunogen
Synthetic peptide corresponding to a region derived from 130-143 amino acids of human vascular endothelial growth factor A
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Formulation
Supplied at 0.9mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3, 0.05% sodium azide and 50% glycerol.
Storage
Store at -20˚C / 1 year
Synonyms
L-VEGF, Vascular endothelial growth factor A, long form, Vascular permeability factor, VEGF, VPF
Suitable Secondaries
Goat Anti-Rabbit IgG H&L Antibody (AP) (A294874)
Goat Anti-Rabbit IgG H&L Antibody (Biotin) (A294795)
Goat Anti-Rabbit IgG H&L Antibody (FITC) (A294887)
Goat Anti-Rabbit IgG H&L Antibody (HRP) (A294888)
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Scientific Validation Data
Validation Data
(3)
Enlarge Image
Anti-VEGFA Antibody (A48157)
Gel: 12% SDS-PAGELane1: Hela, cell lysate Lane2: Jurka cell lysateLane3: 293T cell lysateLane4: 231 cell lysate. Lysates: 40 µg per lane. Primary antibody: 1/450 dilution. Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution. Exposure time: 2 minutes
Enlarge Image
Anti-VEGFA Antibody (A48157)
The image on the left is immunohistochemistry of paraffin-embedded human liver cancer tissue using 31274 (VEGFA Antibody) at dilution 1/25, on the right is treated with the synthetic peptide.
Enlarge Image
Anti-VEGFA Antibody (A48157)
Gel: 12% SDS-PAGELane1: Hela cell lysate Lane2: 293T cell lysateLane3: HepG2 cell lysateLane4: HT29 cell lysateLane5: MDA231 cell lysateLane6: A549 cell lysateLane7: SKOV3 cell lysate. Lysates: 30 µg per lane. Primary antibody: 1/1000 dilution. Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution. Exposure time: 2 minutes
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