Western Blot - Anti-VEGF Receptor 2 (phospho Tyr1175) Antibody [ARC57293] (A309514)
Western blot analysis of A-431 lysates, using Anti-VEGF Receptor 2 (phospho Tyr1175) Antibody [ARC57293] (A309514) at 1:3,000 dilution. A-431 cells were treated by EGF (100 ng/ml) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
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