This antibody reacts with VCP (valosin-containing protein) a 220 kDa intra-acrosomal protein.
Applications
Flow Cytometry, WB, ICC
Dilutions
IHC: 10 µg/ml, membrane permeabilization (acetone) is essential. The antibody Hs-14 is designed for quantitative immunofluorescence analysis of pathological sperms (excellent tool for laboratories of assisted reproduction when optimal method of fertilization is sought), Flow Cytometry: Intraacrosomal staining: 3-12 µg/ml.
Reactivity
Mouse, Human
Immunogen
Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.
Host
Mouse
Clonality
Monoclonal
Clone ID
Hs-14
Isotype
IgM
Conjugate
Unconjugated
Purification
Purified by differential precipitation and solid-phase chromatography.
Concentration
1 mg/ml
Predicted MW
220 kDa
Product Form
Liquid
Formulation
Supplied in Tris Buffered Saline, pH 8, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store at 2-8°C. Do not freeze!
Synonyms
15S Mg(2+)-ATPase p97 subunit, HEL-220, HEL-S-70, TER ATPase, Transitional endoplasmic reticulum ATPase, Valosin-containing protein
Immunofluorescence analysis of VCP in acetone-permeabilized human sperms using Anti-VCP Antibody (A86576) demonstrates its location to the acrosome. (Normal spermiogram shown).
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![Immunocytochemistry - Anti-VCP Antibody [Hs-14] (A86574) - Antibodies.com](https://cdn.antibodies.com/image/catalog/86/A86576_739.jpg?profile=product_top)
![Immunocytochemistry - Anti-VCP Antibody [Hs-14] (A86574) - Antibodies.com](https://cdn.antibodies.com/image/catalog/86/A86576_739.jpg?profile=product_top_thumb)
![Immunocytochemistry - Anti-VCP Antibody [Hs-14] (A86574) - Antibodies.com](https://cdn.antibodies.com/image/catalog/86/A86576_739.jpg?profile=product_image)
![Western Blot - Anti-VCP Antibody [ARC0728] (A307008) - Antibodies.com](https://cdn.antibodies.com/image/catalog/307/A307008_1.jpg?profile=product_alternative)
