Anti-VCP Antibody [Hs-14] (A86576)

Anti-VCP Antibody [Hs-14]

Mouse monoclonal [Hs-14] antibody to VCP.

This antibody reacts with VCP (valosin-containing protein) a 220 kDa intra-acrosomal protein.

Flow Cytometry, WB, ICC

IHC: 10 µg/ml, membrane permeabilization (acetone) is essential. The antibody Hs-14 is designed for quantitative immunofluorescence analysis of pathological sperms (excellent tool for laboratories of assisted reproduction when optimal method of fertilization is sought), Flow Cytometry: Intraacrosomal staining: 3-12 µg/ml.

Mouse, Human

Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.

Mouse

Monoclonal

Hs-14

Purified by differential precipitation and solid-phase chromatography.

1 mg/ml

220 kDa

Liquid

Supplied in Tris Buffered Saline, pH 8, with 15 mM Sodium Azide.

Shipped at 4°C. Store at 2-8°C. Do not freeze!

15S Mg(2+)-ATPase p97 subunit, HEL-220, HEL-S-70, TER ATPase, Transitional endoplasmic reticulum ATPase, Valosin-containing protein