This antibody recognises mouse vascular adhesion molecule 1 (VCAM-1), a cell surface glycoprotein that is also known as CD106. CD106 is expressed predominantly on endothelial cells and expression is up-regulated during inflammation. The ligand for CD106 is the alpha 4 subunit (CD49d) of the integrin VLA-4 (CD49d/CD29). Rat anti Mouse CD106 antibody, clone MVCAM A (429) is reported to partially block VCAM-1 mediated functions.
Applications
Flow Cytometry, IHC-Fr, IF
Dilutions
Flow Cytometry: 1:10 - 1:50, Use 10µl of the suggested working dilution to label 106 cells in 100µl
Reactivity
Mouse
Immunogen
Stromal cell line PA6.
Host
Rat
Clonality
Monoclonal
Clone ID
MVCAM A (429)
Isotype
IgG2a
Conjugate
Unconjugated
Purification
Protein G affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Rat anti Mouse CD106 antibody, clone MVCAM A (429) recognizes murine vascular adhesion molecule 1 (VCAM-1), a cell surface glycoprotein that is also known as CD106. CD106 is expressed predominantly on endothelial cells and expression is up-regulated during inflammation. The ligand for CD106 is the alpha 4 subunit (CD49d) of the integrin VLA-4 (CD49d/CD29).Rat anti Mouse CD106 antibody, clone MVCAM A (429) is reported to partially block VCAM-1 mediated functions (Kinashi and Springer 1994).
Synonyms
CD106, INCAM-100, V-CAM 1, Vascular cell adhesion protein 1, VCAM-1