Varlilumab Biosimilar - Anti-CD27 Antibody - BSA and Azide free
Description
Recombinant human monoclonal antibody to CD27.
Applications
ELISA, Flow Cytometry
Dilutions
ELISA: 1:5,000-10,000, Flow Cytometry: 1:100
Reactivity
Human
Host
Human
Clonality
Monoclonal
Isotype
IgG1
Conjugate
Unconjugated
Purification
Affinity chromatography.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with distilled sterile water.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
General Notes
Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.
ELISA plates were pre-coated with Recombinant Human CD27 Protein (Fc Tag) (A318253) at 2 µg/ml (100 µl/well) which can bind Varlilumab Biosimilar - Anti-CD27 Antibody - BSA and Azide free (A318938) in a linear range of 3.2-80.0 ng/ml. In order to specifically detect Varlilumab Biosimilar - Anti-CD27 Antibody - BSA and Azide free (A318938), Mouse Anti-Human Fab Antibody was used as detection antibody.
ELISA plates were pre-coated with Recombinant Human CD27 Protein (Fc Chimera 6xHis Tag) (A318395) at 2 µg/ml (100 µl/well) which can bind Varlilumab Biosimilar - Anti-CD27 Antibody - BSA and Azide free (A318938) in a linear range of 0.64-16.0 ng/ml.
HEK293 cell line was transfected with: Irrelevant protein (B) and human CD27 (A). Cells were surface stained with 1µg/ml of Varlilumab Biosimilar - Anti-CD27 Antibody - BSA and Azide free (A318938) followed by Anti-Human IgG Antibody (Alexa 488).
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