Anti-V5 Tag Antibody [SV5-Pk5] (A281793)

Anti-V5 Tag Antibody [SV5-Pk5]

Mouse monoclonal [SV5-Pk5] antibody to V5 Tag.

This antibody recognises a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5. Mouse anti V5-Tag antibody, clone SV5-Pk5 has been used to detect recombinant proteins, some of which include transmembrane and secreted proteins, which have been tagged with this epitope. Usually, a 14 amino acid tag has been added to the recombinant proteins, although a smaller epitope of 9 amino acids (that as a peptide inhibit the binding of the monoclonal antibody to its native protein) has also been successfully used. The 14 amino acid epitope is; gly lys pro ile pro asn pro leu leu gly leu asp ser thr. (The 9 amino acid epitope is underlined).

WB, IP, ELISA, IF

WB: 1:100 - 1:500

Viral

A recombinant peptide corresponding to the PK tag fused to a class II - restricted T helper cell epitope.

Mouse

Monoclonal

SV5-Pk5

IgG1

Protein G affinity chromatography of tissue culture supernatant.

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti V5-Tag antibody, clone SV5-Pk5 recognizes a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5. Mouse anti V5-Tag antibody, clone SV5-Pk5 has been used to detect recombinant proteins, some of which include transmembrane and secreted proteins, which have been tagged with this epitope. Usually, a 14 amino acid tag has been added to the recombinant proteins, although a smaller epitope of 9 amino acids (that as a peptide inhibit the binding of the monoclonal antibody to its native protein) has also been successfully used. The 14 amino acid epitope is; gly lys pro ile pro asn pro leu leu gly leu asp ser thr. (The 9 amino acid epitope is underlined).