Utomilumab Biosimilar - Anti-CD137 Antibody - BSA and Azide free
Description
Recombinant human monoclonal antibody to CD137.
Applications
ELISA
Dilutions
ELISA: 1:5,000-10,000
Reactivity
Human
Host
Human
Clonality
Monoclonal
Isotype
IgG2
Conjugate
Unconjugated
Purification
Affinity chromatography.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with distilled sterile water.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
General Notes
Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.
ELISA plates were pre-coated with 2 µg/ml (100 µl/well) Recombinant Human CD137 Protein (Fc Chimera 6xHis Tag) (A318397) which can bind Anti-CD137 Antibody [Utomilumab Biosimilar] - Azide free (A318919) in a linear range of 3.2-400 µg/ml.
Flow cytometry analysis with Anti-CD137 Antibody [Utomilumab Biosimilar] - Azide free (A318919) on Expi293 cells transfected with human 4-1BB (blue histogram) or Expi293 transfected with irrelevant protein (red histogram).
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