Anti-UCP1 Antibody (A15019)

Name: Anti-UCP1 Antibody
Brand: Antibodies.com
SKU: A15019
Price: 355.0 USD
Availability: InStock

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$355

Rabbit polyclonal antibody to UCP1 for WB, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-UCP1 Antibody

Description

Rabbit polyclonal antibody to UCP1.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:100-1:500, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 100-200 of human UCP1 (NP_068605.1).

Sequence

QEFLTAGKETAPSLGSKILAGLTTGGVAVFIGQPTEVVKVRLQAQSHLHGIKPRYTGTYNAYRIIATTEGLTGLWKGTTPNLMRSVIINCTELVTYDLMKE

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

30 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Mitochondrial brown fat uncoupling protein 1, SLC25A7, Solute carrier family 25 member 7, Thermogenin, UCP, UCP 1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-UCP1 Antibody (A15019)

Western blot analysis of Mouse brown adipose tissue, using Anti-UCP1 Antibody (A15019) at 1:400 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Western Blot - Anti-UCP1 Antibody (A15019)

Western blot analysis of Rat brown adipose tissue, using Anti-UCP1 Antibody (A15019) at 1:400 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Immunohistochemistry - Anti-UCP1 Antibody (A15019)

Immunohistochemistry analysis of paraffin-embedded rat brown adipose using Anti-UCP1 Antibody (A15019) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunofluorescence - Anti-UCP1 Antibody (A15019)

Immunofluorescence analysis of rat brown adipose cells using Anti-UCP1 Antibody (A15019) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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