Immunofluorescent analysis of rat hippocampal section stained with Anti-UCH-L1 Antibody [BH7] (A85351), dilution 1:5,000, in green and co-stained with Anti-Fox3 Antibody (A85403), dilution 1:2,000, in red. The blue is Hoechst staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, the brain was post-fixed for 24 hours, cut to 45 µM, and free-floating sections were stained with the above antibodies. The UCH-L1 antibody stains the cell body and dendrites of hippocampal neurons, while the FOX3 antibody labels nuclei of the neuronal cells.
Western blot analysis of tissue lysates using Anti-UCH-L1 Antibody [BH7] (A85351), dilution 1:10,000, in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] pig brain, and [7] pig spinal cord. The single band at 24 kDa corresponds to the UCH-L1 protein.
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