The epitope for this antibody is located in the peptide IQDKEGIPPDQQRLIFAGKQ, amino acids 30-49 of bovine Ubiquitin, with the central 10 amino acid segment, GIPPDQQRLI, likely to be the most significant component of this antibodies epitope.
Anti-Ubiquitin Antibody staining of cerebral cortex of an Alzheimer patient. Neurofibrillary tangles and dystrophic neurites associated with senile plaques stain strongly with this antibody. In the center is a typical neurofibrillary tangle containing neuron.
Western Blot - Anti-Ubiquitin Antibody [Ubi-1] (A85456)
Blots of mono and polyubiquitin (Lane 1), monoubiquitin only (Lane 2), and 100 µg total wet weight of cerebellum, cortex and brain stem respectively (Lane 3-5) were probed with Anti-Ubiquitin Antibody. Material was run out on 20% SDS-PAGE and transferred electrophoretically to PVDF.
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