Anti-Ubiquitin Antibody [Ubi-1] (A85456)

Name: Anti-Ubiquitin Antibody [Ubi-1]
Brand: Antibodies.com
SKU: A85456
Price: 190.0 USD
Availability: InStock

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$190

Mouse monoclonal [Ubi-1] antibody to Ubiquitin for WB, ICC/IF and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Tags:

Lysosome Marker

Specifications

Name

Anti-Ubiquitin Antibody [Ubi-1]

Description

Mouse monoclonal [Ubi-1] antibody to Ubiquitin.

Specificity

The epitope for this antibody is located in the peptide IQDKEGIPPDQQRLIFAGKQ, amino acids 30-49 of bovine Ubiquitin, with the central 10 amino acid segment, GIPPDQQRLI, likely to be the most significant component of this antibodies epitope.

Applications

WB, ICC/IF, IHC

Dilutions

WB: 1:1,000-1:2,000, ICC/IF: 1:2,000, IHC: 1:2,000

Reactivity

Human, Monkey, Horse, Bovine, Porcine, Chicken, Rat, Mouse, Danio, Drosophila, C. elegans

Immunogen

Purified bovine erythrocyte Ubiquitin, coupled to KLH with glutaraldehyde.

Host

Mouse

Clonality

Monoclonal

Clone ID

Ubi-1

Isotype

IgG1

Light Chains

kappa

Conjugate

Unconjugated

Purification

Immunogen affinity purification.

Concentration

1 mg/ml

Molecular Weight

8.5 kDa (monoubiquitin)

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline with 50% Glycerol and 5mM Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Polyubiquitin-B, Polyubiquitin-C, UBB, UBC

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (7)

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Immunohistochemistry - Anti-Ubiquitin Antibody [Ubi-1] (A85456)

Immunohistochemistry analysis of a NBF fixed paraffin embedded human hippocampus section from an Alzheimer’s Disease case. Mouse mAb to ubiquitin, Anti-Ubiquitin Antibody [Ubi-1] (A85456) at a dilution of 1:2,000, was detected in DAB (brown) following the ImmPress method with citra buffer retrieval. Counterstained with Hematoxylin (blue). The Anti-Ubiquitin Antibody [Ubi-1] (A85456) strongly labels flame shapped tangles in pyramidal neurons and dystrophic neurites nuclei characteristic of Alzheimer’s disease. Note: this antibody performs well in testing with 4% PFA or standard NBF fixed human and rat tissues.

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Immunohistochemistry - Anti-Ubiquitin Antibody [Ubi-1] (A85456)

Anti-Ubiquitin Antibody staining of cerebral cortex of an Alzheimer patient. Neurofibrillary tangles and dystrophic neurites associated with senile plaques stain strongly with this antibody. In the center is a typical neurofibrillary tangle containing neuron.

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Immunohistochemistry - Anti-Ubiquitin Antibody [Ubi-1] (A85456)

Immunohistochemistry analysis of a NBF fixed paraffin embedded human hippocampus section from an Alzheimer’s Disease case. Anti-Ubiquitin Antibody [Ubi-1] (A85456) at a dilution of 1:2,000 detected with DAB (brown) using the Vector Labs ImmPRESS method and reagents with citra buffer retrieval. Counterstained with Hematoxylin (blue). The Anti-Ubiquitin Antibody (A85455) antibody strongly labels the cytoplasm of diseased neurons as identified by their morphology.

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Immunohistochemistry - Anti-Ubiquitin Antibody [Ubi-1] (A85456)

Immunohistochemistry analysis of formalin fixed paraffin embedded cerebral cortex of an Alzheimer patient processed with Anti-Ubiquitin Antibody [Ubi-1] (A85456) using HRP/DAB (brown). Counterstained with Hematoxylin (blue). A typical flame shaped tangle is seen in a pyramidal neuron in the center and is surrounded by some dystrophic neurites, also strongly ubiquitin positive. Both are commonly seen in cortical and hippocampal Alzheimer brain sections and are typical for this disease, but are rare or absent in healthy brain.