This antibody detects ubiquitined proteins, including poly-ubiquitinated proteins (K29-, K48-, and K63-linked mono- and polyubiquitinylated proteins). It does not detect free ubiquitin.
Applications
WB, ELISA
Dilutions
WB: 1:1,000, ELISA: 1:1,000
Reactivity
Species Independent
Immunogen
Human Ubiquitin conjugated lysozyme.
Host
Rabbit
Clonality
Monoclonal
Clone ID
FK2
Isotype
IgG
Conjugate
Unconjugated
Purification
Protein A purification.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Western Blot - Anti-Ubiquitin Antibody [FK2] (A305234)
Western blot analysis of Purified poly-ubiquitin chains showing detection of Multiple Ubiquitin protein using Anti-Ubiquitin Antibody [FK2] (A305234) at 1:1,000 for 2 hours at room temperature. Lane 1: Molecular Weight Ladder (MW). Lane 2: K63 Poly Ubiquitin. Lane 3: K48 Poly Ubiquitin. Load: 2 µg. Block: 5% Skim Milk powder in TBST. The secondary antibody used was Goat Anti-Rabbit IgG:HRP at 1:4,000 for 1 hour at room temperature. Color Development: ECL solution for 5 minutes in room temperature. Predicted/Observed Size: Multiple.
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