Anti-U2AF65 Antibody (A13756)

Name: Anti-U2AF65 Antibody
Brand: Antibodies.com
SKU: A13756
Price: 355.0 USD
Availability: InStock

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$355

Rabbit polyclonal antibody to U2AF65 for WB, ICC/IF and IP.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-U2AF65 Antibody

Description

Rabbit polyclonal antibody to U2AF65.

Applications

WB, ICC/IF, IP

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:50-1:100, IP: 1:50-1:200

Reactivity

Human, Mouse

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 192-471 of human U2AF2/U2AF65 (NP_001012496.1).

Sequence

NQDKNFAFLEFRSVDETTQAMAFDGIIFQGQSLKIRRPHDYQPLPGMSENPSVYVPGVVSTVVPDSAHKLFIGGLPNYLNDDQVKELLTSFGPLKAFNLVKDSATGLSKGYAFCEYVDINVTDQAIAGLNGMQLGDKKLLVQRASVGAKNATLSTINQTPVTLQVPGLMSSQVQMGGHPTEVLCLMNMVLPEELLDDEEYEEIVEDVRDECSKYGLVKSIEIPRPVDGVEVPGCGKIFVEFTSVFDCQKAMQGLTGRKFANRVVVTKYCDPDSYHRRDFW

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

54 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

hU2AF(65, hU2AF65, Splicing factor U2AF 65 kDa subunit, U2 auxiliary factor 65 kDa subunit, U2 snRNP auxiliary factor large subunit, U2AF2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-U2AF65 Antibody (A13756)

Western blot analysis of extracts of various cell lines, using Anti-U2AF65 Antibody (A13756) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST.

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Immunofluorescence - Anti-U2AF65 Antibody (A13756)

Immunofluorescence analysis of U2OS cells using Anti-U2AF65 Antibody (A13756). DAPI was used to stain the cell nuclei (blue).

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Western Blot - Anti-U2AF65 Antibody (A13756)

Immunoprecipitation analysis of 200µg extracts of SW620 cells using 1µg of Anti-U2AF65 Antibody (A13756). This Western blot was performed on the immunoprecipitate using Anti-U2AF65 Antibody (A13756) at a dilution of 1:500.

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