Anti-Tyrosine Hydroxylase (phospho Ser40) Antibody (A308525)

Western blot analysis of extracts of PC-12 cells, using Anti-Tyrosine Hydroxylase (phospho Ser40) Antibody (A308525) at 1:1,000 dilution or TH antibody (A0028). PC-12 cells were treated by Forskolin (30 uM) at 37°C for 30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 3s.