Anti-Tyrosine Hydroxylase (phospho Ser40) Antibody (A283960)

Anti-Tyrosine Hydroxylase (phospho Ser40) Antibody

Rabbit polyclonal antibody to Tyrosine Hydroxylase (phospho Ser40).

This antibody recognises tyrosine hydroxylase (TH), also known as tyrosine 3-monooxygenase, when phosphorylated at serine 40.Tyrosine hydroxylase (TH) catalyzes the rate-limiting step in the biosynthetic pathway of the catecholamines dopamine (DA), norepinephrine, and epinephrine. The enzyme exists as a tetramer, with each subunit composed of an N-terminal regulatory domain and a C-terminal catalytic domain.Phosphorylation of TH has been shown to occur at several serine residues. Phosphorylation at serine 40 results in an increase in hydroxylase activity, and phosphorylation at serine 19 is reported to promote phosphorylation of the serine 40 residue. Tyrosine hydroxylase is regularly used as a marker for dopaminergic neurons, which is particularly relevant for research into Parkinson’s disease (Pearson et al 1979).

WB, IHC-Fr, IF

IF: 1:1,000, IHC-Fr: 1:1,000, WB: 1:1,000

Rat, Mouse, Human, Pig

Synthetic phosphopeptide corresponding to an amino acid sequence within Tyrosine Hydroxylase which includes the phosphorylated residue Ser 40.

Rabbit

Polyclonal

IgG

Approximately 60 kDa in PC-12 cell lysates, following stimulation by Okadaic acid.

Liquid

Supplied in 10mM HEPES, pH 7.5, with 0.01% BSA, 50% Glycerol, and 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Rabbit anti Rat tyrosine hydroxylase (pSer40) antibody recognizes tyrosine hydroxylase (TH), also known as tyrosine 3-monooxygenase, when phosphorylated at serine 40.Tyrosine hydroxylase (TH) catalyzes the rate-limiting step in the biosynthetic pathway of the catecholamines dopamine (DA), norepinephrine, and epinephrine. The enzyme exists as a tetramer, with each subunit composed of an N-terminal regulatory domain and a C-terminal catalytic domain.Phosphorylation of TH has been shown to occur at several serine residues. Phosphorylation at serine 40 results in an increase in hydroxylase activity, and phosphorylation at serine 19 is reported to promote phosphorylation of the serine 40 residue (Dunkley et al. 2004). Tyrosine hydroxylase is regularly used as a marker for dopaminergic neurons, which is particularly relevant for research into Parkinson’s disease (Pearson et al 1979).

TH, TYH, Tyrosine 3-hydroxylase, Tyrosine 3-monooxygenase