Anti-Tyrosine Hydroxylase Antibody (A91986)

Western blot analysis of various lysates, using Anti-Tyrosine Hydroxylase Antibody (A91986) at 1:2,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

Immunohistochemistry analysis of paraffin-embedded rat adrenal gland using Anti-Tyrosine Hydroxylase Antibody (A91986) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunofluorescence analysis of Neuro-2alpha cells using Anti-Tyrosine Hydroxylase Antibody (A91986) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

Immunofluorescence analysis of SH-SY5Y cells using Anti-Tyrosine Hydroxylase Antibody (A91986) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).