Anti-Twist Antibody (A15639)

Name: Anti-Twist Antibody
Brand: Antibodies.com
SKU: A15639
Price: 345.0 USD
Availability: InStock

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$345

Rabbit polyclonal antibody to Twist for WB and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Specifications

Name

Anti-Twist Antibody

Description

Rabbit polyclonal antibody to Twist.

Applications

WB, ICC/IF

Dilutions

WB: 1:100-1:500, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 70-150 of human TWIST1 (NP_000465.1).

Sequence

AQGKRGKKSAGCGGGGGAGGGGGSSSGGGSPQSYEELQTQRVMANVRERQRTQSLNEAFAALRKIIPTLPSDKLSKIQTLK

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

21 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

BHLHA38, Class A basic helix-loop-helix protein 38, H-twist, Twist-related protein 1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-Twist Antibody (A15639)

Western blot analysis of extracts of 293T cells, using Anti-Twist Antibody (A15639) at 1:400 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Immunofluorescence - Anti-Twist Antibody (A15639)

Immunofluorescence analysis of HeLa cells using Anti-Twist Antibody (A15639) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).

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Immunofluorescence - Anti-Twist Antibody (A15639)

Immunofluorescence analysis of NIH/3T3 cells using Anti-Twist Antibody (A15639) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).

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