Recombinant rabbit monoclonal [DM141] antibody to TWEAK.
Applications
ELISA, Flow Cytometry
Dilutions
ELISA: 1:5,000-10,000, Flow Cytometry: 1:100
Reactivity
Human
Host
Rabbit
Clonality
Monoclonal
Clone ID
DM141
Isotype
IgG
Conjugate
Unconjugated
Purification
Affinity chromatography.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with distilled sterile water.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
General Notes
Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.
Synonyms
APO3 ligand, APO3L, DR3LG, TNF-related weak inducer of apoptosis, TNFSF12, Tumor necrosis factor ligand superfamily member 12
Anti-TWEAK Antibody [DM141] - BSA and Azide free (A318566)
ELISA plate pre-coated by 1 µg/ml (100 µl/well) Recombinant Human TWEAK Protein (Fc Tag) (A318313) can bind Anti-TWEAK Antibody [DM141] - Azide free (A318566) in a linear range of 0. 1-10 µg/ml.
Flow cytometry analysis with Anti-TWEAK Antibody [DM141] - Azide free (A318566) on Expi293 cells transfected with human TNFSF12 (blue histogram) or Expi293 transfected with irrelevant protein (red histogram).
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