Western blot analysis of extracts of various cell lines, using Anti-Tubulin Antibody (A80593) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Immunofluorescence analysis of NIH/3T3 cells using Anti-Tubulin Antibody (A80593) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).