Western blot analysis of extracts of various cell lines, using Anti-Tubulin Antibody (A80592) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded mouse spleen using Anti-Tubulin Antibody (A80592)Immunohistochemistry analysis of paraffin-embedded Mouse spleen using Alpha-tubulin (ubiquitous) chain antibody (AC024) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-Tubulin Antibody (A80592)Immunohistochemistry analysis of paraffin-embedded Rat kidney using Alpha-tubulin (ubiquitous) chain antibody (AC024) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of NIH/3T3 cells using Anti-Tubulin Antibody (A80592) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
