Anti-TSLP Antibody [H03-5E12] (A280532)

Anti-TSLP Antibody [H03-5E12]

Mouse monoclonal [H03-5E12] antibody to TSLP.

This antibody recognises thymic stromal lymphopoietin (TSLP), a member of the interleukin-2 (IL-2) cytokine family and an IL-7-like cytokine. TSLP plays a role in pathophysiological immunity-related functions. Specifically, TSLP is implicated in allergic reaction, infection, cancer and autoimmunity. During allergic inflammation, the primary producers of TSLP are epithelial cells, keratinocytes, mast cells, smooth muscle cells, dendritic cells and trophoblasts (Takai 2012). TSLP is a ligand of the TSLP receptor (TSLPR), which is expressed by a variety of hematopoietic cell populations and some hematopoietic cells such as epithelial cells. Upon binding of TSLP to TSLPR various signaling pathways can be activated including JAK, STAT, Src kinases and PI3K. Under physiological conditions, induction of these pathways can promote homeostatic polyclonal expansion of T cells in the absence of foreign antigens and drive differentiation of inducible Tregs. The purified Mouse anti Human TSLP antibody, clone H03-5E12 (MCA6284GA) can be used as a capture antibody in a sandwich ELISA with the biotinylated Mouse anti Human TSLP antibody, clone D01-2E2 (MCA6285B) as the detection antibody.

ELISA: 1 µg/ml

Human

E. coli derived recombinant full length human TSLP (Tyr29-Gln159).

Mouse

Monoclonal

H03-5E12

IgG1

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Human TSLP antibody, clone H03-5E12, recognizes thymic stromal lymphopoietin (TSLP), a member of the interleukin-2 (IL-2) cytokine family and an IL-7-like cytokine. TSLP plays a role in pathophysiological immunity-related functions. Specifically, TSLP is implicated in allergic reaction, infection, cancer and autoimmunity (Ziegler et al. 2013). During allergic inflammation, the primary producers of TSLP are epithelial cells, keratinocytes, mast cells, smooth muscle cells, dendritic cells and trophoblasts (Takai 2012). TSLP is a ligand of the TSLP receptor (TSLPR), which is expressed by a variety of hematopoietic cell populations and some hematopoietic cells such as epithelial cells (Ziegler et al. 2013).Upon binding of TSLP to TSLPR various signaling pathways can be activated including JAK, STAT, Src kinases and PI3K. Under physiological conditions, induction of these pathways can promote homeostatic polyclonal expansion of T cells in the absence of foreign antigens and drive differentiation of inducible Tregs (Nakajima et al. 2020). The purified Mouse anti Human TSLP antibody, clone H03-5E12 (MCA6284GA) can be used as a capture antibody in a sandwich ELISA with the biotinylated Mouse anti Human TSLP antibody, clone D01-2E2 (MCA6285B) as the detection antibody.

Thymic stromal lymphopoietin