Anti-TRPC5 Antibody [N67/15] (A304954)

Name: Anti-TRPC5 Antibody [N67/15]
Brand: Antibodies.com
SKU: A304954
Price: 520.0 USD
Availability: InStock

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$520

Mouse monoclonal [N67/15] antibody to TRPC5 for WB, IHC, IP and Antibody Microarray.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Specifications

Name

Anti-TRPC5 Antibody [N67/15]

Description

Mouse monoclonal [N67/15] antibody to TRPC5.

Applications

WB, IHC, IP, Antibody Microarray

Dilutions

WB: 1:1,000, IHC: 1:1,000, ICC/IF: 1:100

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide amino acids 827-845 of human TrpC5 (also known as short transient receptor potential channel 5, and Htrp5).

Host

Mouse

Clonality

Monoclonal

Clone ID

N67/15

Isotype

IgG2b

Conjugate

Unconjugated

Purification

Protein G purification.

Concentration

1 mg/ml

Molecular Weight

~110 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

General Notes

This antibody was formely sold under the Clone ID S67-15.

Synonyms

hTRP-5, hTRP5, Short transient receptor potential channel 5, Transient receptor protein 5, TRP-5, TRP5

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-TRPC5 Antibody [N67/15] (A304954)

Western blot analysis of mouse brain showing detection of 110 kDa TrpC5 protein using Anti-TRPC5 Antibody [N67/15] (A304954) at 1:1,000 for Overnight at 4°C. Lane 1: Molecular Weight Ladder (MW). Lane 2: mouse Brain. Load: 15 ug. Block: 5% Skim Milk powder in TBST. The secondary antibody used was Goat anti-mouse IgG:HRP at 1:7000 for 1 hour at room temperature with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 minutes in room temperature. Predicted/Observed Size: 110 kDa.

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Immunohistochemistry - Anti-TRPC5 Antibody [N67/15] (A304954)

Immunohistochemistry analysis of mouse brain slice, fixed in 10% formalin solution for 12-24 hours at room temperature. The Primary Antibody used was Anti-TRPC5 Antibody [N67/15] (A304954) at 1:1,000 for 1 hour at room temperature. The secondary antibody used was HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at room temperature. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at room temperature. Localization: Nuclear staining. Magnification: 10X.

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Immunofluorescence - Anti-TRPC5 Antibody [N67/15] (A304954)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cells (SH-SY5Y), fixed in 4% PFA for 15 min, using Anti-TRPC5 Antibody [N67/15] (A304954), at 1:50 for overnight at 4°C with slow rocking. The secondary antibody used was AlexaFluor 488 at 1:1,000 for 1 hour at room temperature. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 minutes at room temperature.(A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) TrpC5 Antibody (D) Composite.

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