Anti-TROP2 Antibody [TrMab-6] (A285856)

Western blot analysis of human TROP2 using Anti-TROP2 Antibody [TrMab-6] on lysates of MCF-7, SK-BR-3, and HaCaT cell lines, TROP2-positive and TROP2-negative DU-145 cells, wild-type T-47D, TROP2 knock-out T-47D cells, and TROP2 over-expressing and knock-out MDA-MB-231 cells. Primary antibody concentration was 1 µg/ml.

Separation of A431 cells (red-filled) from human peripheral whole blood cells (black-dashed) in flow cytometry analysis (surface staining) using Anti-TROP2 Antibody [TrMab-6] (concentration in sample is 1.7 µg/ml, GAM-APC).

Immunocytochemistry staining of TROP2 on the surface of unfixed wild-type and TROP2-overexpressing MDA-MB-231 cells with Anti-TROP2 Antibody [TrMab-6] (1 µg/ml), detected with GAM IgG-Alexa Fluor®488 (diluted 1:1000, green); isotype control IgG2b was used as the negative control. DNA stained with DAPI (blue).

Immunohistochemistry staining (paraffin sections) of TROP2 in human and porcine lung tissue with Anti-TROP2 Antibody [TrMab-6] (1:100), detected with En Vision FLEX/HRP. Positive in basolateral parts of airway epithelium. Negative control was stained without primary antibody. Scale bar represents 100 µm.