Anti-TROP2 Antibody (A10307)

Name: Anti-TROP2 Antibody
Brand: Antibodies.com
SKU: A10307
Price: 345.0 USD
Availability: InStock

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$345

Rabbit polyclonal antibody to TROP2 for WB, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Specifications

Name

Anti-TROP2 Antibody

Description

Rabbit polyclonal antibody to TROP2.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 27-270 of human TROP-2 (NP_002344.2).

Sequence

HTAAQDNCTCPTNKMTVCSPDGPGGRCQCRALGSGMAVDCSTLTSKCLLLKARMSAPKNARTLVRPSEHALVDNDGLYDPDCDPEGRFKARQCNQTSVCWCVNSVGVRRTDKGDLSLRCDELVRTHHILIDLRHRPTAGAFNHSDLDAELRRLFRERYRLHPKFVAAVHYEQPTIQIELRQNTSQKAAGDVDIGDAAYYFERDIKGESLFQGRGGLDLRVRGEPLQVERTLIYYLDEIPPKFSM

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

37 - 50 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Cell surface glycoprotein Trop-2, GA733-1, M1S1, Membrane component chromosome 1 surface marker 1, Pancreatic carcinoma marker protein GA733-1, TACSTD2, Tumor-associated calcium signal transducer 2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-TROP2 Antibody (A10307)

Western blot analysis of extracts of various cell lines, using Anti-TROP2 Antibody (A10307) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 1s.

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Western Blot - Anti-TROP2 Antibody (A10307)

Western blot analysis of extracts of Mouse kidney, using Anti-TROP2 Antibody (A10307) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Immunohistochemistry - Anti-TROP2 Antibody (A10307)

Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using Anti-TROP2 Antibody (A10307) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunofluorescence - Anti-TROP2 Antibody (A10307)

Immunofluorescence analysis of A431 cells using Anti-TROP2 Antibody (A10307) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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