Trastuzumab Biosimilar - Anti-ErbB2/HER2 Antibody - BSA and Azide free
Description
Recombinant humanized monoclonal antibody to ErbB2/HER2.
Applications
ELISA, Flow Cytometry
Dilutions
ELISA: 1:5,000-10,000, Flow Cytometry: 1:100
Reactivity
Human
Host
Humanized
Clonality
Monoclonal
Isotype
IgG1
Conjugate
Unconjugated
Purification
Affinity chromatography.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with distilled sterile water.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
General Notes
Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.
Flow cytometry analysis of 15 µg/ml of Trastuzumab Biosimilar - Anti-ErbB2/HER2 Antibody - BSA and Azide free (A318909) on Expi293 cells transfected with Human Her2 protein (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
ELISA plates were pre-coated with Recombinant Human ErbB2/HER2 Protein (6×His Tag) (A318322) at 2 µg/ml (100 µl/well) which can bind Trastuzumab Biosimilar - Anti-ErbB2/HER2 Antibody - BSA and Azide free (A318909) in a linear range of 3.2-400 ng/ml.
ELISA plates were pre-coated with Recombinant Human ErbB2/HER2 Protein (Fc Tag) (A318079) at 2 µg/ml (100 µl/well) which can bind Trastuzumab Biosimilar - Anti-ErbB2/HER2 Antibody - BSA and Azide free (A318909) in a linear range of 3.81-1730.10 ng/ml. In order to specifically detect Trastuzumab Biosimilar - Anti-ErbB2/HER2 Antibody - BSA and Azide free (A318909), Mouse Anti-Human Fab Antibody was used as detection antibody.
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