Anti-Transgelin Antibody [SPM606] (A250075)

Anti-Transgelin Antibody [SPM606]

Mouse monoclonal [SPM606] antibody to Transgelin.

This antibody recognizes a 22kDa protein, identified as Transgelin, also designated SM22-alpha. It may cross-react with SM22-beta. Transgelin is expressed abundantly in smooth muscle cells. The human transgelin gene encodes a 201 amino acid protein that contains nuclear factor-binding motifs known to regulate transcription in smooth muscle. During embryogenesis, transgelin is expressed in smooth, cardiac and skeletal muscle, but is restricted during late fetal development and adulthood to all vascular and visceral smooth muscle cells and low levels of expression in heart. Transgelin is down regulated in several transformed cell lines, indicating that a reduction of transgelin expression may be an early indicator of the onset of transformation. Transgelin also binds Actin, causing Actin fibers to gel within minutes of binding. Binding of transgelin to Actin occurs at a ratio of 1:6 Actin monomers.

Flow Cytometry, IF, IHC-P

Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/ml, IHC-P: 1-2 µg/ml

Human, Bovine, Porcine, Rabbit, Mouse

Recombinant full-length human Transgelin protein.

Mouse

Monoclonal

SPM606

IgG1

kappa

Protein A/G chromatography.

200 µg/ml

22 kDa

Liquid

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

This monoclonal antibody is also available in a different formulation without BSA and Sodium Azide - Anti-Transgelin Antibody [SPM606] - BSA and Azide free (A253255).

22 kDa actin-binding protein, Protein WS3-10, SM22, SM22-alpha, Smooth muscle protein 22-alpha, TAGLN, WS3-10