Mouse monoclonal [MEM-75] antibody to Transferrin Receptor (PerCP-Cyanine 5.5).
Specificity
This antibody reacts with an extracellular epitope of CD71 antigen, a 95 kDa type II homodimeric transmembrane glycoprotein expressed on activated B and T lymphocytes, macrophages, and erythroid precursors. It is lost on resting blood leukocytes. This antibody does not block binding of transferrin to the receptor.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 4 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
NALM-6 human pre-B cell line.
Host
Mouse
Clonality
Monoclonal
Clone ID
MEM-75
Isotype
IgG1
Conjugate
PerCP-Cyanine 5.5
Excitation: 482nm, Emission: 695nm
Purification
This antibody is conjugated with tandem dye PerCP-Cyanine 5.5 under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Predicted MW
95 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Flow cytometric analysis of CD71 in K562 cells with Anti-CD71 Antibody [MEM-75] (PerCP-Cyanine 5.5).
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