Anti-Transferrin Antibody [HTF-14] (A280668)

$480

Mouse monoclonal [HTF-14] antibody to Transferrin for ELISA, WB, IF and Functional Assays.

Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 4-6 business days.
Name
Anti-Transferrin Antibody [HTF-14]
Description
Mouse monoclonal [HTF-14] antibody to Transferrin.
Specificity
This antibody recognises the N-terminal domain of human transferrin and has been shown to block binding of transferrin to the transferrin receptor (CD71). Clone HTF-14 is not able to recognize receptor bound transferrin. Clone HTF-14 recognises a conformational epitope in a loop of the N lobe of transferrin involving the critical Lys-144 residue, also essential for binding of the N lobe to the transferrin receptor Transferrin is a 77 kDa plasma protein synthesized by the liver involved with the transport of iron. Each transferrin molecule has two domains, both with facility to carry 2 ferric ions. The iron/transferrin complex is essential for hemoglobin synthesis and certain types of cell division.
Applications
ELISA, WB, IF, Functional Assays
Reactivity
Human, Pig, Rabbit, Rat
Cross Reactivity
This antibody does not cross react with Sheep, Bovine, Dog, or Horse
Immunogen
Purified porcine transferrin.
Host
Mouse
Clonality
Monoclonal
Clone ID
HTF-14
Isotype
IgG1
Conjugate

Unconjugated

Purification
Protein A affinity chromatography of ascites.
Concentration
1 mg/ml
Molecular Weight
Approximately 77 kDa under reducing conditions in human plasma.
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with <0.1% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Mouse anti transferrin antibody, clone HTF-14 recognizes the N-terminal domain of human transferrin and has been shown to block binding of transferrin to the transferrin receptor (CD71). Clone HTF-14 is not able to recognize receptor bound transferrin. Clone HTF-14 recognizes a conformational epitope in a loop of the N lobe of transferrin involving the critical Lys-144 residue, also essential for binding of the N lobe to the transferrin receptor (Mason et al.2009)Transferrin is a 77 kDa plasma protein synthesized by the liver involved with the transport of iron. Each transferrin molecule has two domains, both with facility to carry 2 ferric ions. The iron/transferrin complex is essential for hemoglobin synthesis and certain types of cell division.
Synonyms
Beta-1 metal-binding globulin, Serotransferrin, Siderophilin, TF
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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